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Bias caused by using different isolation media for assessing the genetic diversity of a natural microbial population

TitleBias caused by using different isolation media for assessing the genetic diversity of a natural microbial population
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2000
AuthorsTabacchioni, Silvia, Chiarini L., Bevivino Annamaria, Cantale Cristina, and Dalmastri Claudia
JournalMicrobial Ecology
Volume40
Pagination169 - 176
Date Published2000///
Abstract

The influence of isolation medium on the biodiversity of Burkholderia cepacia strains recovered from the rhizosphere of Zea mays was evaluated by comparing the genetic diversity of isolates obtained by plating serial dilutions of root macerates on the two selective media TB-T and PCAT. From each medium, 50 randomly chosen colonies were isolated. On the basis of the restriction patterns of DNA coding for 16S rRNA (16S rDNA) amplified by means of PCR (ARDRA), all strains isolated from TB-T medium were assigned to the B. cepacia species, whereas among PCAT isolates only 74% were assigned to the B. cepacia species. Genetic diversity among the PCAT and TB-T isolates was evaluated by the random amplified polymorphic DNA (RAPD) technique. The analysis of molecular variance (AMOVA) method was applied to determine the variance component for RAPD patterns. Most of the genetic diversity (90.59%) was found within the two groups of isolates, but an appreciable amount (9.41%) still separated the two groups (P < 0.001). Mean genetic distances among PCAT isolates (10.39) and TB-T isolates (9.36) were significantly different (P < 0.0001). The results indicate that the two different isolation media select for B. cepacia populations with a different degree of genetic diversity. Moreover, a higher degree of genetic diversity was observed among strains isolated from PCAT medium than among those isolated from TB-T medium.

Notes

Cited By (since 1996): 26Export Date: 26 August 2010Source: Scopus

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0033652044&partnerID=40&md5=00767a08f504374b0adbb54191f8b5ee
Citation Key410