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Lactiplantibacillus plantarum monolayer enhanced bactericidal action of carvacrol: biofilm inhibition of viable foodborne pathogens and spoilage microorganisms

TitleLactiplantibacillus plantarum monolayer enhanced bactericidal action of carvacrol: biofilm inhibition of viable foodborne pathogens and spoilage microorganisms
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2023
AuthorsPoscente, Valeria, Di Gregorio Luciana, Costanzo Manuela, Nobili Chiara, Bernini Roberta, Garavaglia Luigi, and Bevivino Annamaria
JournalFrontiers in Microbiology
Volume14
Type of ArticleArticle
ISSN1664302X
Keywordsantimicrobial activity, article, bactericidal activity, bacterium, biofilm, carvacrol, cell viability, colony formation, controlled study, drug efficacy, Flow cytometry, food control, food industry, food spoilage, foodborne pathogen, lactiplantibacillus plantarum, Listeria monocytogenes, nonhuman, prevalence
Abstract

The prevalence of biofilm-associated microorganisms and the increasing use of ready-to-eat fresh products represent the current duality the food industry must address. Innovative and eco-friendly antibiofilm solutions and appropriate microbiological food control systems are urgently needed to improve food quality and safety. This study aimed to investigate the in vitro combined efficacy of carvacrol with a pre-formed biofilm monolayer of the probiotic Lactiplantibacillus plantarum DSM 20174. The antimicrobial activity of carvacrol against both planktonic and sessile cells of foodborne pathogens and spoilage microorganisms, alone or in the presence of the pre-formed biofilm of L. plantarum, was investigated by culture-based methods along with flow cytometry (FCM) to monitor cells' cultivability and viability. The synergistic action of carvacrol and the pre-formed biofilm of L. plantarum was evaluated in the 96-well plates. The results showed that L. plantarum pre-formed biofilm monolayer enhanced the antimicrobial effect of carvacrol determining a bactericidal action while the treatment alone induced the viable but not culturable (VBNC) cell state only. Furthermore, the great efficacy of the combined treatment allowed the application of a lower concentration of carvacrol (100 ppm) to achieve significant damage in cell viability. In conclusion, the incorporation of carvacrol into the L. plantarum pre-formed biofilm represents a promising alternative for an antimicrobial functionalized ready-to-eat packaging. Copyright © 2023 Poscente, Di Gregorio, Costanzo, Nobili, Bernini, Garavaglia and Bevivino.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85178881313&doi=10.3389%2ffmicb.2023.1296608&partnerID=40&md5=d4045b3f1a7af6b862e72dd92b350f34
DOI10.3389/fmicb.2023.1296608
Citation KeyPoscente2023