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Bacterial community and proteome analysis of fresh-cut lettuce as affected by packaging.

TitleBacterial community and proteome analysis of fresh-cut lettuce as affected by packaging.
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2016
AuthorsDi Carli, Mariasole, De Rossi P., Paganin Patrizia, Del Fiore Antonella, Lecce Francesca, Capodicasa Cristina, Bianco Linda, Perrotta Gaetano, Mengoni Alessio, Bacci Giovanni, Daroda Lorenza, Dalmastri Claudia, Donini Marcello, and Bevivino Annamaria
JournalFEMS microbiology letters
Date Published2016 Jan
KeywordsBacteria, bacterial count, bacterium, biota, chemistry, classification, Colony Count, Electrophoresis, Food packaging, Genomics, isolation and purification, Lettuce, Mass Spectrometry, Microbial, microbiological examination, Microbiological Techniques, microbiology, Polymorphism, proteome, Proteomics, Restriction Fragment Length, restriction fragment length polymorphism

With the growing demand of fresh-cut vegetables, a variety of packaging films are produced specifically to improve safety and quality of the fresh vegetables over the storage period. The aim of our work was to evaluate the influence of different packaging films on the quality of fresh-cut lettuce analyzing changes in bacterial community composition and modifications at the proteome level, by means of culture-dependent/culture-independent methods and differential gel electrophoresis combined with mass spectrometry analysis. Total viable counts indicated the presence of a highly variable and complex microbial flora, around a mean value of 6.26 log10 CFU g(-1). Analysis of terminal-restriction fragment length polymorphism data indicated that bacterial communities changed with packaging films and time, showing differences in community composition and diversity indices between the commercially available package (F) and the new packages (A and C), in the first days after packaging. Also proteomic analysis revealed significant changes, involving proteins related to energy metabolism, photosynthesis, plant defense and oxidative stress processes, between F and A/C packages. In conclusion, microbiological and proteomic analysis have proved to be powerful tools to provide new insights into both the composition of leaf-associated bacterial communities and protein content of fresh-cut lettuce during the shelf-life storage process.


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Citation Key5477