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Transportomics for the Characterization of Plant Apocarotenoid Transmembrane Transporters

TitleTransportomics for the Characterization of Plant Apocarotenoid Transmembrane Transporters
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2020
AuthorsDemurtas, Olivia Costantina, R. Francisco de Brito, Martinoia E., and Giuliano Giovanni
JournalMethods in molecular biology (Clifton, N.J.)
Keywordsapocarotenal, Biological Transport, Carotenoid, Carotenoids, carrier protein, Chromatography, electroporation, genetics, high performance liquid chromatography, High Pressure Liquid, Liquid, liquid chromatography, Mass Spectrometry, Membrane Transport Proteins, metabolism, microsome, Microsomes, Plant, Plants, procedures, Proteomics, transport at the cellular level, Yeast, Yeasts

Apocarotenoids are carotenoid derivatives produced by the nonenzymatic or enzymatic cleavage of carotenoids, followed by different enzymatic modifications. In plants, apocarotenoids play different roles, such as attraction of pollinators and seeds dispersal, defense against pathogens and herbivores, protection against photo-oxidative stresses, stimulation and inhibition of plant growth and regulation of biological processes in the case of phytohormones abscisic acid and strigolactones. While carotenoids are in general plastid-localized metabolites, apocarotenoids can reach different final destinations inside or outside the cell. The mechanisms of apocarotenoid transport through biological membranes have been poorly studied. This chapter describes a method to characterize transmembrane transporters involved in the transport of polar and amphipathic apocarotenoids. This protocol was successfully used to in vitro characterize the transport activity of ATP-binding cassette (ABC) and multidrug and toxic extrusion (MATE) in microsomes isolated from Saccharomyces cerevisiae expressing these plant transporters.


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Citation KeyDemurtas202089