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Flavonoid metabolism in Forsythia flowers

TitleFlavonoid metabolism in Forsythia flowers
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication1998
AuthorsRosati, C., Cadic A., Duron M., Amiot M.-J., Tacchini M., Martens S., and Forkmann G.
JournalPlant Science
Keywordsarticle, enzyme activity, flavonoid, Forsythia giraldiana, Forsythia intermedia, Forsythia ovata, gene expression regulation, high performance liquid chromatography, metabolism, oxidoreductase, phytochemistry, transgenic plant

The flavonoid pathway metabolism was studied in petals and sepals of Forsythia X intermedia cv. 'Spring Glory'. The activities of the flavonoid enzymes chalcone synthase (CHS), flavanone 3-hydroxylase (FHT) and flavonol synthase (FLS) were measured. The dihydroflavonol 4-reductase (DFR) role was also studied by comparing flavonoid accumulation in transgenic plants for a heterologous DFR gene and wild-type F. X intermedia cv. 'Spring Glory', already investigated for DFR gene expression and activity. HPLC analyses complemented enzymatic investigations, showing that: (i) rutin (quercetin 3- rutinoside) is the major flavonol accumulated in petals and sepals (ca. 90% of the flavonol pool) and; (ii) quercetin and cyanidin derivatives are the exclusive flavonols and anthocyanins in sepals, respectively. The overall data demonstrated that the flavonoid pathway in F. X intermedia flower organs leads to the major accumulation of 3',4'-dihydroxylated compounds, and that 3'-hydroxylation of the B-ring occurs mainly at flavonoid intermediate stage(s). Comparative HPLC analyses of F. X intermedia cv. 'Spring Glory' and three other genotypes (F. giraldiana, F. X intermedia 'Korfor' Goldzauber® and F. ovata 'Robusta') confirmed the major production of flavonols by Forsythia flavonoid metabolism and suggested a method for screening Forsythia genotypes based on anthocyanin accumulation pattern in sepals.


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Citation KeyRosati1998133