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Metabolic engineering of xanthophyll content in tomato fruits

TitleMetabolic engineering of xanthophyll content in tomato fruits
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2002
AuthorsDharmapuri, S., Rosati C., Pallara Patrizia, Aquilani R., Bouvier F., Camara B., and Giuliano Giovanni
JournalFEBS Letters
KeywordsArabidopsis, article, beta carotene, beta carotene hydroxylase, beta cryptoxanthin, Blotting, carotene, Carotenoid, cryptoxanthin, Dominant, enzyme assay, fruit, fruit ripening, Gene expression, Genes, Genetic engineering, Genetically Modified, Intramolecular Lyases, lycopene, lycopene beta cyclase, Lycopersicon esculentum, metabolic engineering, Mixed Function Oxygenases, Phenotype, plant leaf, Plant leaves, Plants, priority journal, promoter region, protein expression, Reverse Transcriptase Polymerase Chain Reaction, reverse transcription polymerase chain reaction, Tomato, transgene, Transgenes, unclassified drug, Western, Western blotting, xanthophyll, Xanthophylls, Zeaxanthin

Ripe tomato fruits accumulate significant amounts of the linear carotene lycopene, but only trace amounts of xanthophylls (oxygenated carotenoids). We overexpressed the lycopene β-cyclase (b-Lcy) and β-carotene hydroxylase (b-Chy) genes under the control of the fruit-specific Pds promoter. Transgene and protein expression was followed through semi-quantitative reverse transcription-PCR, Western blotting, and enzyme assays. Fruits of the transformants showed a significant increase of β-carotene, β-cryptoxanthin and zeaxanthin. The carotenoid composition of leaves remained unaltered. The transgenes and the phenotype are inherited in a dominant Mendelian fashion. This is the first example of successful metabolic engineering of xanthophyll content in tomato fruits. © 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.


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Citation KeyDharmapuri200230