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Characterization of a temperature-sensitive carrot cell mutant impaired in somatic embryogenesis

TitleCharacterization of a temperature-sensitive carrot cell mutant impaired in somatic embryogenesis
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication1988
AuthorsSchiavo, F.L., Giuliano Giovanni, and Sung Z.R.
JournalPlant Science
Volume54
Pagination157 - 164
Date Published1988///
KeywordsDaucus carota, developmental mutant, Protoplast fusion
Abstract

Among the various carrot cell mutants, temperature-sensitive for embryogenesis, line ts59, blocked at the globular stage, was characterized. A biological characterization indicates that the function missing or altered in ts59 is necessary two times during development: the first, 5 days after the onset of embryogenesis and the second, at the transition between heart- and torpedo-shaped embryos. Fusion experiments of protoplasts of ts59 with a wild-type line, inactivated with iodoacetate, indicate dominance of the ts59 mutation. A biochemical analysis was carried out of various embryonic markers of ts59 and compared with wild-type. A remarkable alteration was found in the pattern of heat-shock proteins whose expression was shown to vary during development. © 1988.

Notes

Cited By (since 1996): 3Export Date: 8 November 2010Source: Scopus

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0342482568&partnerID=40&md5=286bb0ad649784b6736dc4baa12e926a
Citation Key714