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2-D DIGE analysis of UV-C radiation-responsive proteins in globe artichoke leaves

Title2-D DIGE analysis of UV-C radiation-responsive proteins in globe artichoke leaves
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2012
AuthorsFalvo, S., Di Carli Mariasole, Desiderio Angiola, Benvenuto Eugenio, Moglia A., America T., Lanteri S., and Acquadro A.
JournalProteomics
Volume12
Pagination448 - 460
Date Published2012
ISBN Number16159853 (ISSN)
Keywords2-D DIGE, abiotic stress, Artichoke, article, Asteraceae, chaperone, chloroplast protein, Cynara scolymus, Electrophoresis, energy metabolism, Gel, gene expression regulation, Globe artichoke, Leaf proteome, liquid chromatography, Mass Spectrometry, Molecular Sequence Annotation, nonhuman, photosynthesis, Plant, plant leaf, Plant leaves, Plant Proteins, plant proteomics, priority journal, proteasome, protein analysis, Protein Folding, protein function, protein interaction, protein localization, Protein-protein network, proteome, Tandem Mass Spectrometry, two dimensional difference gel electrophoresis, Two-Dimensional, ultraviolet C radiation, Ultraviolet Rays, UV-C stress, vegetable protein
Abstract

Plants respond to ultraviolet stress inducing a self-defence through the regulation of specific gene family members. The UV acclimation is the result of biochemical and physiological processes, such as enhancement of the antioxidant enzymatic system and accumulation of UV-absorbing phenolic compounds (e.g. flavonoids). Globe artichoke is an attractive species for studying the protein network involved in UV stress response, being characterized by remarkable levels of inducible antioxidants. Proteomic tools can assist the evaluation of the expression patterns of UV-responsive proteins and we applied the difference in-gel electrophoresis (DIGE) technology for monitoring the globe artichoke proteome variation at four time points following an acute UV-C exposure. A total of 145 UV-C-modulated proteins were observed and 119 were identified by LC-MS/MS using a \~144000 customized Compositae protein database, which included about 19000 globe artichoke unigenes. Proteins were Gene Ontology (GO) categorized, visualized on their pathways and their behaviour was discussed. A predicted protein interaction network was produced and highly connected hub-like proteins were highlighted. Most of the proteins differentially modulated were chloroplast located, involved in photosynthesis, sugar metabolisms, protein folding and abiotic stress. The identification of UV-C-responsive proteins may contribute to shed light on the molecular mechanisms underlying plant responses to UV stress. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Notes

Cited By :3Export Date: 16 July 2015CODEN: PROTCCorrespondence Address: Acquadro, A.; DIVAPRA, University of Turin, Via L. da Vinci 44, 10095 Grugliasco (TO), Italy; email: alberto.acquadro@unito.itChemicals/CAS: proteasome, 140879-24-9; Plant ProteinsReferences: Stapleton, A.E., Ultraviolet-radiation and plants - burning questions (1992) Plant Cell, 4, pp. 1353-1358;Jenkins, G.I., Signal transduction in responses to UV-B radiation (2009) Annu. Rev. Plant Biol., 60, pp. 407-431; Björn, L.O., McKenzie, R.L., (2008) Photobiology: The Science of Life and Light, pp. 503-530. , Ozone depletion and the effects of ultraviolet radiation, in: Björn, L. O., Ed.), Springer, New York; Ballare, C.L., Rousseaux, M.C., Searles, P.S., Zaller, J.G., Impacts of solar ultraviolet-B radiation on terrestrial ecosystems of Tierra del Fuego (southern Argentina) - an overview of recent progress (2001) J. Photochem. Photobiol. 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