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Design of a Diagnostic Immunoassay for Aflatoxin M1 Based on a Plant-Produced Antibody

TitleDesign of a Diagnostic Immunoassay for Aflatoxin M1 Based on a Plant-Produced Antibody
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2022
AuthorsCapodicasa, Cristina, Bastiani Erica, Serra Thea, Anfossi Laura, and Catellani Marcello
Type of ArticleArticle
KeywordsAflatoxin M1, animal, Animals, Antibodies, Antibody, chemistry, enzyme linked immunosorbent assay, Enzyme-Linked Immunosorbent Assay, food contamination, Immunoassay, milk

A new green competitive ELISA for aflatoxin M1 quantification in raw milk was developed. This diagnostic tool is based on an anti AFM1 mAb produced by plant molecular farming in alternative to classical systems. Our assay, showing an IC50 below 25 ng/L, fits with the requirements of EU legislation limits for AFM1 (50 ng/L). Optimal accuracy was achieved in correspondence of the decision levels (25 and 50 ng/L), and the assay enabled AFM1 quantification in the range 5–110 ng/L, with limit of detection 3 ng/L. Moreover, to evaluate a real applicability in diagnostics, raw milk-spiked samples were analysed, achieving satisfactory recovery rates of AFM1. In conclusion, an efficient and ready-to-use diagnostic assay for the quantification of aflatoxin M1 in milk, based on a plant-produced recombinant mAb, has been successfully developed. © 2022 by the authors.


Cited by: 0; All Open Access, Gold Open Access, Green Open Access

Citation KeyCapodicasa2022
PubMed ID36548748