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NOD2 Is Regulated by MIR-320 in Physiological Conditions but this Control Is Altered in Inflamed Tissues of Patients with Inflammatory Bowel Disease

TitoloNOD2 Is Regulated by MIR-320 in Physiological Conditions but this Control Is Altered in Inflamed Tissues of Patients with Inflammatory Bowel Disease
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2016
AutoriPierdomenico, Maria, Cesi Vincenzo, Cucchiara S., Vitali Roberta, Prete E., Costanzo Manuela, Aloi M., Oliva S., and Stronati L.
RivistaInflammatory Bowel Diseases
Volume22
Paginazione315-326
ISSN10780998
Parole chiave3' untranslated region, adolescent, article, azathioprine, binding site, Blotting, case control study, Case-Control Studies, caspase recruitment domain protein 15, cell culture, Cells, Child, colitis, comparative study, controlled study, corticosteroid, Crohn disease, Cultured, cytokine, Cytokines, down regulation, enzyme activation, enzyme activity, enzyme immunoassay, ex vivo study, Female, Fluorescent Antibody Technique, follow up, Follow-Up Studies, gene expression regulation, genetic transcription, genetic transfection, genetics, HT 29 cell line, HT-29 cell line, HT29 Cells, human, human cell, human tissue, Humans, Immunity, Immunoenzyme Techniques, immunoglobulin enhancer binding protein, immunology, in vitro study, inflammation, Innate, innate immunity, interleukin 8, intestine biopsy, low drug dose, luciferase, male, mesalazine, Messenger, messenger RNA, metabolism, methotrexate, microRNA, microRNA 320a, microRNA 320b, microRNA 320c, microRNA 320d, MicroRNAs, MIRN320 microRNA, NOD2 protein, Nod2 Signaling Adaptor Protein, Pathology, Preschool, preschool child, priority journal, prognosis, protein analysis, protein expression, protein function, protein targeting, real time polymerase chain reaction, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, reverse transcription polymerase chain reaction, RNA, tissue level, tumor necrosis factor alpha, Ulcerative, ulcerative colitis, unclassified drug, upregulation, Western, Western blotting
Abstract

Background: Large evidence supports the role of microRNAs as new important inflammatory mediators by regulating both the adaptive and innate immunity. In the present study, we speculated that miR-320 controls NOD2 (nucleotide-binding oligomerization domain) expression, because it contains multiple binding sites in the 3′-untranslated region of the gene. NOD2, the first gene associated to increased susceptibility to Crohn's disease, is a cytosolic receptor that senses wall peptides of bacteria and promotes their clearance through initiation of a proinflammatory transcriptional program. This study aims at demonstrating that NOD2 is a target of miR-320 as well as investigating the role of inflammation in modulating the miR-320 control on NOD2 expression and analyzing miR-320 expression in intestinal biopsies of children with inflammatory bowel disease. Methods: The colonic adenocarcinoma cell line HT29 was used to assess the miR-320-mediated regulation of NOD2 expression. MiR-320 and NOD2 expression were analyzed in mucosal samples of 40 children with inflammatory bowel disease. Results: During inflammation, NOD2 expression is inversely correlated with miR-320 expression in vitro and ex vivo. Exogenous miR-320 transfection in HT29 cells leads to a significant decrease of NOD2 expression, whereas the miR-320 inhibitor transfection leads to increase of NOD2 expression, nuclear translocation of nuclear factor B, and activation of downstream cytokines. Conclusions: We show for the first time that NOD2 expression is under the control of miR-320. We also show in vitro and ex vivo that inflammation induces a decrease of miR-320 and the latter correlates negatively with NOD2 expression. © 2016 Crohn's & Colitis Foundation of America, Inc.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84955613204&doi=10.1097%2fMIB.0000000000000659&partnerID=40&md5=8cf451f57ef48be9a40b50fa0e6a8390
DOI10.1097/MIB.0000000000000659
Citation KeyPierdomenico2016315