Sorry, you need to enable JavaScript to visit this website.

Sperm DNA fragmentation induced by DNAse I and hydrogen peroxide: an in vitro comparative study among different mammalian species.

TitoloSperm DNA fragmentation induced by DNAse I and hydrogen peroxide: an in vitro comparative study among different mammalian species.
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2010
AutoriVillani, Paola, Eleuteri Patrizia, Grollino Maria Giuseppa, Rescia Michele, Altavista Pierluigi, Spanò M., Pacchierotti Francesca, and Cordelli Eugenia
RivistaReproduction
Volume140
Issue3
Paginazione445-52
Data di pubblicazione2010 Sep
ISSN17417899
Parole chiaveAnimals, Cattle, chromatin assembly and disassembly, Comet Assay, Deoxyribonuclease I, DNA fragmentation, Dose-Response Relationship, Drug, Humans, Hydrogen Peroxide, In Situ Nick-End Labeling, male, Mice, Mice, Inbred C57BL, Oxidants, Oxidative stress, Spermatozoa
Abstract

Sperm DNA damage may have adverse effects on reproductive outcome. Sperm DNA breaks can be detected by several tests, which evaluate DNA integrity from different and complementary perspectives and offer a new class of biomarkers of the male reproductive function and of its possible impairment after environmental exposure. The remodeling of sperm chromatin produces an extremely condensed nuclear structure protecting the nuclear genome from adverse environments. This nuclear remodeling is species specific, and differences in chromatin structure may lead to a dissimilar DNA susceptibility to mutagens among species. In this study, the capacity of the comet assay in its two variants (alkaline and neutral) to detect DNA/chromatin integrity has been evaluated in human, mouse, and bull sperm. The hypothesis that chromatin packaging might influence the amount of induced and detectable DNA damage was tested by treating sperm in vitro with DNAse I, whose activity is strictly dependent upon its DNA accessibility. Furthermore, hydrogen peroxide (H2O2) was used to assess whether spermatozoa of the three species showed a different sensitivity to oxidative stress. DNAse I-induced damage was also assessed by the sperm chromatin structure assay and the TUNEL assay, and the performances of these two assays were compared and correlated with the comet assay results. Results showed a different sensitivity to DNAse I treatment among the species with human sperm resulting the most susceptible. On the contrary, no major differences among species were observed after H2O2 treatment. Furthermore, the three tests show a good correlation in revealing sperm with DNA strand breaks.

Note

cited By 29

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-77956221743&doi=10.1530%2fREP-10-0176&partnerID=40&md5=d44fbfaa298ea361c6a70802210ed418
DOI10.1530/REP-10-0176
Alternate JournalReproduction
Citation Key4911
PubMed ID20584992