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Chromogenic in situ hybridization to detect HER-2/neu gene amplification in histological and ThinPrep®-processed breast cancer fine-needle aspirates: A sensitive and practical method in the trastuzumab era

TitoloChromogenic in situ hybridization to detect HER-2/neu gene amplification in histological and ThinPrep®-processed breast cancer fine-needle aspirates: A sensitive and practical method in the trastuzumab era
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2006
AutoriVocaturo, A., Novelli Flavia, Benevolo M., Piperno G., Marandino F., Cianciulli A.M., Merola R., Donnorso R.P., Sperduti I., Buglioni S., and Mottolese M.
RivistaOncologist
Volume11
Paginazione878-886
ISSN10837159
Parole chiaveAntibodies, article, aspiration biopsy, Biopsy, Breast cancer, Breast Neoplasms, Chromogenic Compounds, chromogenic in situ hybridization, chromogenic substrate, controlled study, cytology, diagnostic accuracy, drug efficacy, erbB-2, Female, Fine-Needle, fluorescence, fluorescence in situ hybridization, gene amplification, gene overexpression, Histocytological Preparation Techniques, histopathology, human, human cell, human tissue, Humans, Immunoenzyme Techniques, immunohistochemistry, In Situ Hybridization, intermethod comparison, major clinical study, Monoclonal, oncogene neu, Papanicolaou test, paraffin, Paraffin Embedding, priority journal, Prospective Studies, Receptor, Retrospective Studies, Sensitivity and Specificity, trastuzumab
Abstract

The increasing evidence of trastuzumab efficacy in breast cancer (BC) patients means that an accurate and reproducible evaluation of HER-2 status is of paramount importance in histological and in cytological samples. Currently, the two main methods used to analyze HER-2 amplification or overexpression are fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC). Although the two methods are strongly correlated for histological tissue, the evaluation of tumor morphology through FISH may be difficult and fluorescence fades quickly. These limitations can be overcome by chromogenic in situ hybridization (CISH), which can visualize the amplification product along with morphological features. In view of this, in the present study, we analyzed the usefulness of CISH on formalin-fixed, paraffin-embedded (FFPE) BC specimens and investigated whether CISH can be a valid technique in the determination of HER-2 status for fine-needle aspirates (FNAs) processed by liquid-based cytology. The results we obtained in a retrospective series of 111 FFPE BC specimens demonstrated good concordance between CISH and IHC and between CISH and FISH. The former concordance was comparable with that observed between FISH and IHC. When CISH was applied to a prospective series of 53 FNAs, from surgically removed BC, our data showed evidence of a higher concordance of results between liquid-based cytology and the companion FFPE tissues using CISH rather than HercepTest™. Therefore, CISH analysis, which is a valuable and reproducible alternative to FISH for selecting breast cancer patients for trastuzumab therapy, can lower false-positive immunocytochemistry findings in ThinPrep®-processed FNAs. ©AlphaMed Press.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-33748555196&doi=10.1634%2ftheoncologist.11-8-878&partnerID=40&md5=aaab4e4dbec7a6001244035502d74255
DOI10.1634/theoncologist.11-8-878
Citation KeyVocaturo2006878