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In situ Raman microspectroscopic identification and localization of carotenoids: Approach to monitoring of UV-B irradiation stress on Antarctic fungus

TitoloIn situ Raman microspectroscopic identification and localization of carotenoids: Approach to monitoring of UV-B irradiation stress on Antarctic fungus
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2000
AutoriArcangeli, Caterina, and Cannistraro S.
RivistaBiopolymers - Biospectroscopy Section
Volume57
Paginazione179-186
ISSN00063525
Parole chiaveanalytic method, Antarctic fungus, Antarctic Regions, article, beta carotene, Biological membranes, Carotenoid, Carotenoids, cell membrane, Cell Wall, chemical bond, Fungal, Fungi, fungus, Mitosporic Fungi, Nondestructive examination, nonhuman, Pigments, protein analysis, protein localization, Raman, Raman microspectroscopy, Raman spectrometry, Raman spectroscopy, reproducibility, signal noise ratio, Spectrum Analysis, Spores, ultraviolet B radiation, Ultraviolet radiation, Ultraviolet Rays
Abstract

The in situ Raman microspectroscopic properties of an Antarctic fungus are investigated to assess the nature and the spatial localization of the main chromophores and to study their spectral changes under enhanced UV-B irradiation. The Raman spectroscopic features of spores in situ are consistent with those of carotenoid-like pigments. In particular, the Raman shifts seem to be related either to the frequency modes of long conjugated double-bond carotenoids or to protein bound β-carotene. The spectroscopic analysis at different spore depths clearly shows the strongest Raman signal arises from cell wall and membrane structures. The intensity of such a signal shows a drastic reduction upon UV-B irradiation without any significant frequency change. The use of Raman microspectroscopy for nondestructively monitoring the UV-B effects on Arthrobotrys ferox spores is also discussed. (C) 2000 John Wiley and Sons, Inc. The in situ Raman microspectroscopic properties of an Antarctic fungus are investigated to assess the nature and the spatial localization of the main chromophores and to study their spectral changes under enhanced UV-B irradiation. The Raman spectroscopic features of spores in situ are consistent with those of carotenoid-like pigments. In particular, the Raman shifts seem to be related either to the frequency modes of long conjugated double-bond carotenoids or to protein bound β-carotene. The spectroscopic analysis at different spore depths clearly shows the strongest Raman signal arises from cell wall and membrane structures. The intensity of such a signal shows a drastic reduction upon UV-B irradiation without any significant frequency change. The use of Raman microspectroscopy for nondestructively monitoring the UV-B effects on Arthrobotrys ferox spores is also discussed.

Note

cited By 21

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0034019706&doi=10.1002%2f%28SICI%291097-0282%282000%2957%3a3%3c179%3a%3aAID-BIP6%3e3.0.CO%3b2-4&partnerID=40&md5=6dee13693a452a279d420d9d4dd2bcb7
DOI10.1002/(SICI)1097-0282(2000)57:3<179::AID-BIP6>3.0.CO;2-4
Citation KeyArcangeli2000179