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Construction of chromosome-specific DNA libraries covering the whole genome of field bean (Vicia faba L.)

TitoloConstruction of chromosome-specific DNA libraries covering the whole genome of field bean (Vicia faba L.)
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione1996
AutoriMacas, J., Gualberti G., Nouzová M., Samec P., Lucretti Sergio, and Doležel Jaroslav
RivistaChromosome Research
Volume4
Paginazione531 - 539
Data di pubblicazione1996///
Parole chiaveChromosome flow sorting, Chromosome-specific, Degenerate oligonucleotide-primed polymerase chain reaction, DNA libraries, Genome mapping, Vicia faba
Abstract

Recombinant DNA libraries were constructed for seven chromosome types isolated from two translocation lines of field bean (Vicia faba L.) with reconstructed karyotypes. The chromosomes were selected so that the set of libraries covers the whole V. faba genome more than once. Individual chromosome types were highly purified by flow sorting, and their DNA was amplified by degenerate oligonucleotide-primed (DOP) polymerase chain reaction (PCR) and cloned into a plasmid vector. The choice of restriction site present in PCR primer and refinement of cloning protocol resulted in high cloning efficiency and allowed generation of libraries consisting of about 106 clones from 250 or 1000 sorted chromosomes. The insert size ranged between 50 and 2200 bp and the mean length estimated in individual libraries varied between 310 and 487 bp. Hybridization of cloned fragments with labelled genomic DNA showed that about 60% of inserts represented unique or low-copy sequences. The suitability of the libraries far genome mapping was demonstrated by isolation of clones containing microsatellite motifs.

Note

Cited By (since 1996): 31Export Date: 26 August 2010Source: Scopus

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0029860735&partnerID=40&md5=3fbcb57811f25bce587f2202d6cd8307
Citation Key448