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Adventitious shoot regeneration from leaf explants and stem nodes of Lilium

TitoloAdventitious shoot regeneration from leaf explants and stem nodes of Lilium
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2003
AutoriBacchetta, Loretta, Remotti P.C., Bernardini C., and Saccardo F.
RivistaPlant Cell, Tissue and Organ Culture
Volume74
Paginazione37 - 44
Data di pubblicazione2003///
Parole chiaveBulblet, Leaf regeneration, Lilium spp., Micropropagation, Nodal stem culture, TDZ
Abstract

A method for the regeneration of lily plantlets (Lilium spp.) through different morphogenic pathways is described. Plant regeneration was obtained from in vitro cultured leaves of four lily hybrids, cultured on Murashige and Skoog’s basal medium supplemented with cytokinins (TDZ and BA) and auxins (NAA and IBA) at different concentrations. Direct shoot regeneration occurred with all tested media for the Asiatic lilies ’Elite’ and ’Pollyanna’ and also for the Oriental hybrid ’Star Gazer’. Callus developed on TDZ-enriched medium from leaf segments of L. longiflorum cv. ’Snow Queen’ regenerated by direct organogenesis. This occurred on a medium with auxin/cytokinin balance which was lower than other genotypes. There were fewer problems of sterilization with leaves from sprouted bulbs than in vitro scale culture. This suggests that the leaf-segments obtained in this way could be an alternative to the scales as a source of material for propagation. A protocol for micropropagation based on bulblets from in vitro shoot-tip-derived stem nodes was also used. The development of pseudo-bulbets is particularly advantageous since it allows for structures characterised by absent or low dormancy. Regenerated shoots have been rooted and successfully acclimatized to greenhouse conditions where they flowered after the second year giving plants with true-to-type shape and colour.

Note

Cited By (since 1996): 24Export Date: 25 August 2010Source: Scopus

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0038219971&partnerID=40&md5=5dce3b79399eb49241c39d82a07f23f4
Citation Key255