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Bacterial metabarcoding by 16S rRNA gene ion torrent amplicon sequencing.

TitoloBacterial metabarcoding by 16S rRNA gene ion torrent amplicon sequencing.
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2015
AutoriFantini, Elio, Gianese Giulio, Giuliano Giovanni, and Fiore Alessia
RivistaMethods in molecular biology (Clifton, N.J.)
Volume1231
Paginazione77-90
Data di pubblicazione2015
ISSN1940-6029
Parole chiave16S, Agar Gel, agar gel electrophoresis, amplicon, article, Bacteria, Bacteria (microorganisms), Bacterial, bacterial gene, bacterial genome, bacterial metabarcoding, bacterial RNA, bacterium, biochip, chemistry, chromosome map, Chromosome Mapping, devices, DNA, DNA barcoding, DNA Primers, DNA sequence, Electrophoresis, Gene Library, Genes, genetics, Genome, genome analysis, Genomic Library, high throughput sequencing, High-Throughput Nucleotide Sequencing, ion torrent amplicon sequencing, metagenomics, Microbial diversity, molecular genetics, Molecular Sequence Annotation, nonhuman, Polymerase Chain Reaction, primer DNA, procedures, Productivity, proton, Protons, reference database, Ribosomal, RNA, RNA 16S, RNA sequence, Sequence Analysis, statistics and numerical data, synthesis, Taxonomic, taxonomic rank
Abstract

Ion Torrent is a next generation sequencing technology based on the detection of hydrogen ions produced during DNA chain elongation; this technology allows analyzing and characterizing genomes, genes, and species. Here, we describe an Ion Torrent procedure applied to the metagenomic analysis of 16S rRNA gene amplicons to study the bacterial diversity in food and environmental samples.

Note

cited By 10

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84914126910&doi=10.1007%2f978-1-4939-1720-4_5&partnerID=40&md5=dc54c53bd87f805ea2717f3012ab7a26
DOI10.1007/978-1-4939-1720-4_5
Citation Key4936