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In vitro and in vivo toxicity evaluation of plant virus nanocarriers.

TitoloIn vitro and in vivo toxicity evaluation of plant virus nanocarriers.
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2015
AutoriBlandino, Agnese, Lico Chiara, Baschieri Selene, Barberini Lanfranco, Cirotto Carlo, Blasi Paolo, and Santi Luca
RivistaColloids Surf B Biointerfaces
Volume129
Paginazione130-6
Data di pubblicazione2015 May 01
ISSN18734367
Parole chiaveAnimals, chick embryo, Chickens, Drug Carriers, Genetic engineering, Genetic Vectors, hemolysis, In Vitro Techniques, Mice, Mice, Inbred C57BL, Nanoparticles, nanotechnology, Plasmids, Potexvirus, teratogenesis, Tobacco, Tombusvirus
Abstract

The use of biological self-assembling materials, plant virus nanoparticles in particular, appears very intriguing as it allows a great choice of symmetries and dimensions, easy chemical and biological engineering of both surface and/or internal cavity as well as safe and rapid production in plants. In this perspective, we present an initial evaluation of the safety profile of two structurally different plant viruses produced in Nicotiana benthamiana L. plants: the filamentous Potato virus X and the icosahedral Tomato bushy stunt virus. In vitro haemolysis assay was used to test the cytotoxic effects, which could arise by pVNPs interaction with cellular membranes, while early embryo assay was used to evaluate toxicity and teratogenicity in vivo. Data indicates that these structurally robust particles, still able to infect plants after incubation in serum up to 24h, have neither toxic nor teratogenic effects in vitro and in vivo. This work represents the first safety-focused characterization of pVNPs in view of their possible use as drug delivery carriers.

DOI10.1016/j.colsurfb.2015.03.039
Alternate JournalColloids Surf B Biointerfaces
Citation Key6041
PubMed ID25847457